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层粘连蛋白γ3(LAMC3)重组蛋白

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产品名称: 层粘连蛋白γ3(LAMC3)重组蛋白
产品型号: LAMC3
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

层粘连蛋白γ3(LAMC3)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。层粘连蛋白γ3(LAMC3)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


层粘连蛋白γ3(LAMC3)重组蛋白  的详细介绍

层粘连蛋白γ3(LAMC3)重组蛋白


  • 物种Mus musculus (Mouse,小鼠)
  • 性状冻干粉内**水平<1.0EU/g(LAL法测定)
  • 亚细胞定位n/a
  • 预测分子量14.7kDa
  • 实际分子量-(差异分析请参阅说明书)
  • 片段与标签Gly751~Cys875 (Accession # Q9R0B6) with N-terminal His Tag
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 纯度> 95%
  • 等电点-
  • 应用SDS-PAGE; WB; ELISA; IP
    层粘连蛋白γ3(LAMC3)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
     
     
    层粘连蛋白γ3(LAMC3)重组蛋白
    Laminin Gamma 3 (LAMC3) 
    Organism: Mus musculus (Mouse) 
     Instruction manual 
     
    FOR IN VITRO USE AND RESEARCH USE ONLY 
    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 
     
    5th Edition (Revised in January, 2013) 
     
    层粘连蛋白γ3(LAMC3)重组蛋白[ DESCRIPTION ] 
    Protein Names: Laminin Gamma 3 
    Synonyms: LAMC3 
    Species: Mouse 
    Size: 100g 
    Source: Escherichia coli-derived 
    Subcellular Location: Secreted, extracellular space, 
    extracellular matrix, basement membrane. 
    [ PROPERTIES ] 
    Residues: Gly751~Cys875 (Accession # Q9R0B6), 
    with N-terminal His-Tag. 
    Grade & Purity: >95%, 17kDa as determined by 
    SDS-PAGE reducing conditions. 
    Formulation: Supplied as lyophilized form in PBS, pH 
    7.4, containing 5% sucrose, 0.01% sarcosyl. 
    Endotoxin Level: <1.0 EU per 1g (determined by the 
    LAL method). 
    Applications: SDS-PAGE; WB; ELISA; IP. 
    (May be suitable for use in other assays to be determined by the end user.) 
    Predicted Molecular Mass: 14.7kDa 
    Predicted isoelectric point: 6.2 
    Mouse LAMC3  
     
     
    层粘连蛋白γ3(LAMC3)重组蛋白[ PREPARATION ] 
    Reconstitute in sterile PBS, pH7.2-pH7.4. 
    [ STORAGE AND STABILITY ] 
    Storage: Avoid repeated freeze/thaw cycles. 
     Store at 2-8oC for one month. 
     Aliquot and store at -80oC for 12 months. 
    Stability Test: The thermal stability is described by the loss rate of the target 
    protein. The loss rate was determined by accelerated thermal degradation test, 
    that is, incubate the protein at 37oC for 48h, and no obvious degradation and 
    precipitation were observed. (Referring from China Biological Products Standard, 
    which was calculated by the Arrhenius equation.) The loss of this protein is less 
    than 5% within the expiration date under appropriate storage condition. 
    [ SEQUENCES ] 
    The target protein is fused with N-terminal His-Tag, its sequence is listed below. 
    MGHHHHHHSGSEF- GFYGNAFSGR ADDCQPCPCP GQSACATIPE SGDVVCTHCP 
    PGQRGRRCES CEDGFFGDPL GLSGAPQPCR RCQCSGNVDL NAVGNCDPHS 
    GHCLRCLYNT TGAHCEHCRE GFYGSAVATR PVDKC 
     
     
     
     
     
     
     



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