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表皮生长因子受体(EGFR)重组蛋白

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产品名称: 表皮生长因子受体(EGFR)重组蛋白
产品型号: EGFR
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

表皮生长因子受体(EGFR)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。表皮生长因子受体(EGFR)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


表皮生长因子受体(EGFR)重组蛋白  的详细介绍

表皮生长因子受体(EGFR)重组蛋白


  • 物种Homo sapiens (Human,人)
  • 性状冻干粉内**水平<1.0EU/g(LAL法测定)
  • 亚细胞定位Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Golgi apparatus membrane; Endosome. Endosome membrane. Secrete
  • 预测分子量37.5kDa
  • 实际分子量-(差异分析请参阅说明书)
  • 片段与标签Lys713~Asp984 (Accession # P00533) with N-terminal His Tag
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 纯度> 95%
  • 等电点6.7
  • 应用SDS-PAGE; WB; ELISA; IP
    表皮生长因子受体(EGFR)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    表皮生长因子受体(EGFR)重组蛋白
    Recombinant Epidermal Growth Factor Receptor (EGFR)
    Organism Species: Homo sapiens (Human)
    Instruction manual
    FOR IN VITRO USE AND RESEARCH USE ONLY
    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
    9th Edition (Revised in Jul, 2013)
    表皮生长因子受体(EGFR)重组蛋白[ PROPERTIES ]
    Residues: Lys713~Asp984 (Accession # P00533),
    with two N-terminal Tags, His-tag and S-tag. Host: E. coli
    Subcellular Location: Cell membrane;
    Single-pass type I membrane protein.
    Endoplasmic reticulum membrane; Golgi
    apparatus membrane; Endosome. Endosome
    membrane. Secreted. Purity: >95%
    Endotoxin Level: <1.0EU per 1μg
    (determined by the LAL method).
    Formulation: Supplied as lyophilized form in PBS,
    pH7.4, containing 5% trehalose, 0.01% sarcosyl.
    Predicted isoelectric point: 6.7
    Predicted Molecular Mass: 37.5kDa
    Applications: SDS-PAGE; WB; ELISA; IP.
    (May be suitable for use in other assays to be determined by the end user.)[ USAGE ]
    Reconstitute in sterile PBS, pH7.2-pH7.4.
    表皮生长因子受体(EGFR)重组蛋白[ STORAGE AND STABILITY ]
    Storage: Avoid repeated freeze/thaw cycles.
    Store at 2-8oC for one month.
    Aliquot and store at -80oC for 12 months.
    Stability Test: The thermal stability is described by the loss rate of the target
    protein. The loss rate was determined by accelerated thermal degradation test,
    that is, incubate the protein at 37oC for 48h, and no obvious degradation and
    precipitation were observed. (Referring from China Biological Products Standard,
    which was calculated by the Arrhenius equation.) The loss of this protein is less
    than 5% within the expiration date under appropriate storage condition.
    [ SEQUENCES ]
    The target protein is fused with two N-terminal Tags, His-tag and S-tag, its
    sequence is listed below.
    MH HHH HHS SG LVP R GSGMKE TAAA KFE RQ H MDS P D LG T DD DDKAMA DIGS
    EFELRRQAC- KKIKVLGS GAFGTVYKGL WIPEGEKVKI PVAIKELREA TSPKANKEIL
    DEAYVMASVD NPHVCRLLGI CLTSTVQLIT QLMPFGCLLD YVREHKDNIG SQYLLNWCVQ
    IAKGMNYLED RRLVHRDLAA RNVLVKTPQH VKITDFGLAK LLGAEEKEYH AEGGKVPIKW
    MALESILHRI YTHQSDVWSY GVTVWELMTF GSKPYDGIPA SEISSILEKG ERLPQPPICT
    IDVYMIMVKC WMIDADSRPK FRELIIEFSK MARDPQRYLV IQGD



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