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半乳糖凝集素3(GAL3)重组蛋白

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产品名称: 半乳糖凝集素3(GAL3)重组蛋白
产品型号: GAL3
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

半乳糖凝集素3(GAL3)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。半乳糖凝集素3(GAL3)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


半乳糖凝集素3(GAL3)重组蛋白  的详细介绍

半乳糖凝集素3(GAL3)重组蛋白

  • 物种Homo sapiens (Human,人)
  • 性状冻干粉内**水平<1.0EU/g(LAL法测定)
  • 亚细胞定位Cytoplasm. Nucleus. Secreted
  • 预测分子量27.4kDa
  • 实际分子量-(差异分析请参阅说明书)
  • 片段与标签Met1~Ile250 (Accession # P17931) with N-terminal His Tag
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 纯度> 95%
  • 等电点8.6
  • 应用SDS-PAGE; WB; ELISA; IP
    半乳糖凝集素3(GAL3)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    半乳糖凝集素3(GAL3)重组蛋白
    Recombinant Galectin 3 (GAL3)
    Organism Species: Homo sapiens (Human)
    Instruction manual
    FOR IN VITRO USE AND RESEARCH USE ONLY
    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
    9th Edition (Revised in Jul, 2013)
    半乳糖凝集素3(GAL3)重组蛋白[ PROPERTIES ]
    Residues: Met1~Ile250 (Accession # P17931), with
    N-terminal His-Tag.
    Host: E. coli
    Subcellular Location: Cytoplasm. Nucleus.
    Secreted.
    Purity: >95%
    Endotoxin Level: <1.0EU per 1μg
    (determined by the LAL method).
    Formulation: Supplied as lyophilized form in PBS,
    pH7.4, containing 5% sucrose, 0.01% sarcosyl.
    Predicted isoelectric point: 8.6
    Predicted Molecular Mass: 27.4kDa
    Applications: SDS-PAGE; WB; ELISA; IP.
    (May be suitable for use in other assays to be determined by the end user.)
    半乳糖凝集素3(GAL3)重组蛋白[ USAGE ]
    Reconstitute in sterile PBS, pH7.2-pH7.4.
    kDa
    94
    66.2
    45
    33
    26
    20
    14.4
    15% SDS-PAGE[ STORAGE AND STABILITY ]
    Storage: Avoid repeated freeze/thaw cycles.
    Store at 2-8oC for one month.
    Aliquot and store at -80oC for 12 months.
    Stability Test: The thermal stability is described by the loss rate of the target
    protein. The loss rate was determined by accelerated thermal degradation test,
    that is, incubate the protein at 37oC for 48h, and no obvious degradation and
    precipitation were observed. (Referring from China Biological Products Standard,
    which was calculated by the Arrhenius equation.) The loss of this protein is less
    than 5% within the expiration date under appropriate storage condition.
    [ SEQUENCES ]
    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
    MGHHHHHHSGS-MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS
    YPGAY PG QAP PGAYPG QA PP GAYP GAP GAY PGAPAP GVY P GP PS GP GAYP
    SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ
    RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK
    VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
    [ REFERENCES ]
    1. Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17. 2. Haudek K.C., et al. (2010) Biochim. Biophys. Acta 1800:181-189. 3. Henderson N.C., Sethi T. (2009) Immunol. Rev. 230:160-171. 4. Martins L., et al. (2006) Arq Bras Endocrinol Metabol 50:1075-1081.


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