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脂解激活脂蛋白受体(LSR)重组蛋白

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产品名称: 脂解激活脂蛋白受体(LSR)重组蛋白
产品型号: LSR
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

脂解激活脂蛋白受体(LSR)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。脂解激活脂蛋白受体(LSR)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的蛋白质成为重组蛋白,重组蛋白在生物信号传导过程,**研发,科学研究中扮演着重要角色。


脂解激活脂蛋白受体(LSR)重组蛋白  的详细介绍

  • 物种脂解激活脂蛋白受体(LSR)重组蛋白Homo sapiens (Human,人)
  • 宿主来源原核表达
  • E.coli
  • 内**水平<1.0EU/g(LAL法测定)
  • 亚细胞定位细胞膜
  • 预测分子量33.9kDa
  • 实际分子量23kDa(差异分析请参阅说明书)
  • 片段与标签Met1~Gly259 with N-terminal His Tag
  • 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
  • 性状冻干粉
  • 纯度> 95%
  • 等电点7.1
  • 应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling

 脂解激活脂蛋白受体(LSR)重组蛋白用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

脂解激活脂蛋白受体(LSR)重组蛋白

Recombinant Lipolysis Stimulated Lipoprotein Receptor (LSR)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Met1~Gly259

Tags: N-terminal His-Tag

Tissue Specificity: Testis, Kidney, Liver. Subcellular Location: Cell membrane; Single-pass membrane protein. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

脂解激活脂蛋白受体(LSR)重组蛋白Predicted isoelectric point: 7.1

Predicted Molecular Mass: 33.9kDa

Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as

follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex.

脂解激活脂蛋白受体(LSR)重组蛋白[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE



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