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Ⅳ型胶原(COL4)检测试剂盒

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产品名称: Ⅳ型胶原(COL4)检测试剂盒
产品型号: 48T/96T盒
产品展商: YBscience
产品文档: 无相关文档

简单介绍

Ⅳ型胶原(COL4)检测试剂盒用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、细胞培养上清、组织匀浆等本标本。产品种类齐全、质量可靠、价格优惠、灵敏度高、效果稳定、易保存、操作简单。


Ⅳ型胶原(COL4)检测试剂盒  的详细介绍

Ⅳ型胶原(COL4)检测试剂盒ELISA Kit for Collagen Type IV (COL4)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. YBA180Mu
Sample type Serum, plasma and other biological fluids
Format 96T
Assay length 4.5 hours
Detection range 0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.37ng/mL.
Ⅳ型胶原(COL4)检测试剂盒Specificity
This assay has high sensitivity and excellent specificity for detection of Collagen Type IV (COL4). 
No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Collagen Type IV (COL4) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type IV (COL4) in samples. 
Matrix Recovery range (%) Average(%)
serum(n=5) 88-102 96
EDTA plasma(n=5) 86-102 93
heparin plasma(n=5) 80-89 81
Ⅳ型胶原(COL4)检测试剂盒Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV (COL4) were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV (COL4) were tested on 3 different plates, 8 replicates in each plate. 
CV(%) = SD/meanX100 
Intra-Assay: CV<10% 
Inter-Assay: CV<12% 
Ⅳ型胶原(COL4)检测试剂盒Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type IV (COL4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. 
Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 85-99% 80-93% 79-91%
EDTA plasma(n=5) 82-96% 80-99% 83-101% 97-105%
heparin plasma(n=5) 78-98% 88-96% 86-95% 93-101%
Ⅳ型胶原(COL4)检测试剂盒Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. 
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Ⅳ型胶原(COL4)检测试剂盒Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120L Assay Diluent A 1×12mL
Detection Reagent B 1×120L Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Ⅳ型胶原(COL4)检测试剂盒Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100L standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100L prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100L prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90L Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50L Stop Solution. Read at 450nm imtely. 
Ⅳ型胶原(COL4)检测试剂盒Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV (COL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV (COL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV (COL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV (COL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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